Professor Jimmy Botella
Professor
School of Agriculture and Food Sustainability
+61 7 336 51128
John Hines Building (62), Room 428, St Lucia campus
Researcher biography
Dr Botella's research interests are in genetic engineering, molecular biology and signal transduction in plants.
Dr. Jimmy Botella is Professor of Plant Biotechnology at the University of Queensland. He obtained a degree in Quantum Chemistry from the University of Madrid (Spain) and a PhD in Biochemistry from the University of Malaga (Spain). After postdoctoral positions at Michigan State University and Pennsylvania State University he joined the University of Queensland in 1995. At UQ he founded the Plant Genetic Engineering Laboratory (PGEL) specialising in the fields of tropical and subtropical agricultural biotechnology for almost 15 years. J. Botella has eleven international patents in the field of Plant Biotechnology and is a founding member of two biotechnology companies (Coridon Ltd. and Origo Biotech).
Dr Botella is a member of the Plant Molecular Biology and Biotechnology research group.
Some highlights of the Plant Genetic Engineering Laboratory's research:
- Production and field trial of the world's first genetically modified pineapples with genetic constructs to control flowering time. These pineapples will allow farmer control over harvesting times.
- Development of a new technology to confer protection against nematode infestation.
- Discovery of a gene that can confer resistance to the devastating fungus Fusarium oxysporum in plants.
- Development of a new technology to confer protection against pathogenic fungi in plants.
Research interests
Dr. Botella's research has two major foci: basic cell biology and applied biotechnology. In cell biology he is interested in studying the function of the Heterotrimeric G proteins in plants. This family of proteins is extremely important in humans but their role in pant systems is still largely unknown. Dr. Botella's research has strongly contributed to the current body of knowledge available in plants with critical contributions such as the discovery and characterization of the first plant gamma subunits and the establishment of these subunits as the critical element conferring function specificity to all plant G proteins. Dr. Botella's team has also discovered the important role that these proteins play in defense against pathogens. New and unpublished data has now revealed that G proteins are important yield enhancing factors in crops such as rice. Another research interest resides in the communication between plants and insects. There is plenty of knowledge of how important smell, volatiles emitted by the plant, is for foraging insects in order to determine their host preferences. Nevertheless, most of the available studies have been performed using synthetic chemicals in artificial experimental settings. Dr. Botella's team and collaborators have genetically engineered plants to produce different volatile mixes in the flowers in order to perform in vivo behavioral studies in insects.
Biotechnology research at the Plant Genetic Engineering Laboratory mostly arises from discoveries made in basic research. The PGEL focuses in tropical and subtropical crops. These crops have attracted little attention in terms of biotechnology but are essential sources of food and energy for a large part of the world's population, especially in Asia and the Indian subcontinent. The PGEL has developed a number of platform technologies that can be applied to multiple crops in order to confer resistance to pathogens, modify plant architecture and control flowering time.
Current research projects include:
- Plant heterotrimeric G proteins: New roles in defence, stomatal control and ABA perception.
- Putting smells into context: using in vivo technologies to understand plant-insect odour communication.
- Use of host-derived RNA interference technology to control plant pathogens (especially pathogenic fungi and nematodes).
- Control of Fusarium wilt disease.
- Genetic improvement of grain crops.
- Genetic engineering research projects
Book Chapters
Maruta, Natsumi, Trusov, Yuri and Botella, Jose R. (2016). Yeast three-hybrid system for the detection of protein-protein interactions. In Jose R. Botella and Miguel A. Botella (Ed.), Plant signal transduction Second edition ed. (pp. 145-154) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3115-6_12
Trusov, Yuri, Dietzgen, Ralf G., Maruta, Natsumi and Botella, Jose R. (2016). Simplified assays for evaluation of resistance to Alternaria brassicicola and Turnip Mosaic Virus. In Botella, JR and Botella, MA (Ed.), Plant Signal Transduction (pp. 219-228) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3115-6_18
Botella, Jose R. and Botella, Miguel A. (2016). Preface. Plant Signal Transduction Methods and Protocols. (pp. VII-X) New York, NY, United States: Humana Press. doi: 10.1007/978-1-4939-3115-6
Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2013). Fusarium oxysporum infection assays in Arabidopsis. G protein-coupled receptor signaling in plants: methods and protocols. (pp. 67-72) edited by Mark P. Running. New York, United States: Humana Press. doi: 10.1007/978-1-62703-532-3_7
Trusov, Yuri, Jorda, Lucia, Molina, Antonio and Botella, Jose Ramon (2010). G Proteins and Plant Innate Immunity. Integrated G Proteins Signaling in Plants. (pp. 221-250) edited by Shaul Yalovsky, Frantisek Baluska and Alan Jones. Netherlands: Springer. doi: 10.1007/978-3-642-03524-1
Brosnan, C.A., McCallum,E.J., Botella, J.R. and Carroll, B.J. (2008). RNA Interference. The Handbook of Plant Functional Genomics: Concepts and Protocols. (pp. 209-225) edited by Kahl, G. and Meksem, K.. Germany: Wiley - Blackwell. doi: 10.1002/9783527622542.ch10
Botella, J.R. and Smith, M. (2008). Genomics of Pineapple, Crowning the King of Tropical Fruits. Plant Genetics/Genomics: Genomics of Tropical Crop Plants. (pp. 441-451) edited by Moore, P.H. and Ming, R.. United States: Springer.
Smith, M. K., Ko, H. L., Sanewski, G. M. and Botella, J. R. (2005). Bromeliaceae. Biotechnology of Fruit and Nut Crops. (pp. 157-172) edited by R. E. Litz. Cambridge, USA: CABI Publishing.
Botella, J. R. (2003). Biotechnology and reduced spoilage. Food Preservation Techniques. (pp. 243-262) edited by Peter Zeuthen and Leif Bogh-Sorensen. Cambridge, United Kingdom: Woodhead Publishing Limited. doi: 10.1016/B978-1-85573-530-9.50017-6
Botella, J. R. (2002). Genes selected for their role in modifying post-harvest life. Fruit and Vegetable Biotechnology. (pp. 136-151) edited by Victoriano Valpuesta. Cambridge, England: Woodhead Publishing Ltd. doi: 10.1533/9781855736412.1.136
Journal Articles
Wu, Chengyun, Wang, Xingsong, Li, Yan, Zhen, Weibo, Wang, Chunfei, Wang, Xiaoqing, Xie, Zhouli, Xu, Xiumei, Guo, Siyi, Botella, José Ramón, Zheng, Binglian, Wang, Wei, Song, Chun-Peng and Hu, Zhubing (2024). Sequestration of DBR1 to stress granules promotes lariat intronic RNAs accumulation for heat-stress tolerance. Nature Communications, 15 (1) 7696, 1-17. doi: 10.1038/s41467-024-52034-w
Pang, Xinyue, Sun, Jiaju, Jia, Jingyu, Trusov, Yuri, Chandora, Rahul, Botella, José Ramon, Li, Xin and Gu, Shaobin (2024). Integration of single-cell and spatial RNA sequencing uncovers spatiotemporal transition of fruit senescence trajectory from exocarp to mesocarp in Pitaya (Hylocereus undatus). Postharvest Biology and Technology, 213 112954, 1-12. doi: 10.1016/j.postharvbio.2024.112954
Gong, Zheng, Previtera, Dominic Andrew, Wang, Yijie and Botella, José Ramón (2024). Geminiviral-induced genome editing using miniature CRISPR/Cas12j (CasΦ) and Cas12f variants in plants. Plant Cell Reports, 43 (3) 71, 71. doi: 10.1007/s00299-023-03092-9
Karimian, Parastoo, Trusov, Yuri and Botella, Jose Ramon (2024). Conserved Role of Heterotrimeric G Proteins in Plant Defense and Cell Death Progression. Genes, 15 (1) ARTN 115, 115. doi: 10.3390/genes15010115
Zou, Yiping, Mason, Michael Glenn and Botella, Jose Ramon (2023). A low-cost, portable, dual-function readout device for amplification-based point-of-need diagnostics. Applied and Environmental Microbiology, 89 (12) A6, 1-17. doi: 10.1128/aem.00902-23
Guo, Xiaopeng, Zhang, Xin, Jiang, Shan, Qiao, Xin, Meng, Bolun, Wang, Xiaohang, Wang, Yanan, Yang, Kaihuan, Zhang, Yilan, Li, Na, Chen, Tianyan, Kang, Yiyang, Yao, Mengyi, Zhang, Xuan, Wang, Xinru, Zhang, Erling, Li, Junhua, Yan, Dawei, Hu, Zhubing, Botella, José Ramón, Song, Chun-Peng, Li, Yunhai and Guo, Siyi (2023). E3 ligases MAC3A and MAC3B ubiquitinate UBIQUITIN-SPECIFIC PROTEASE14 to regulate organ size in Arabidopsis. Plant Physiology, 194 (2), 684-697. doi: 10.1093/plphys/kiad559
Burman, Sriti, Mason, Michael G., Hintzsche, Jessica, Zou, Yiping, Gibbs, Lucy, MacGillycuddy, Laura, Magarey, Robert C. and Botella, José R. (2023). Changing the diagnostic paradigm for sugarcane: development of a mill-based diagnostic for ratoon stunting disease in crude cane juice. Frontiers in Plant Science, 14 1257894, 1-13. doi: 10.3389/fpls.2023.1257894
Li, Liangliang, Li, Linxiao, Cui, Shenghui, Qian, Dongdong, Lyu, Shijie, Liu, Wencheng, Botella, José Ramón, Li, Huilin, Burritt, David J., Tran, Lam-Son Phan, Li, Weiqiang and Zhang, Yu (2023). PDC1 is activated by ABF4 and inhibits seed germination by promoting ROS accumulation in Arabidopsis. Environmental and Experimental Botany, 206 105188, 105188. doi: 10.1016/j.envexpbot.2022.105188
Zhou, Yusen, Zhang, Xiaomin, Chen, Jing, Guo, Xiaopeng, Wang, Hongyan, Zhen, Weibo, Zhang, Junli, Hu, Zhubing, Zhang, Xuebing, Botella, José Ramón, Ito, Toshiro and Guo, Siyi (2022). Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana. BMC Plant Biology, 22 (1) 248, 1-12. doi: 10.1186/s12870-022-03622-9
Zhang, Zhengjing, Zeng, Wenjie, Zhang, Wenxin, Li, Jing, Kong, Dali, Zhang, Lei, Wang, Rui, Peng, Fangnan, Kong, Zhe, Ke, Yongping, Zhang, Heng, Kim, Chanhong, Zhang, Huiming, Botella, Jose Ramón, Zhu, Jian-Kang and Miki, Daisuke (2022). Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis. Plant Physiology, 190 (4), 2203-2216. doi: 10.1093/plphys/kiac431
Botella, José R. (2022). Point-of-care DNA amplification for disease diagnosis and management. Annual Review of Phytopathology, 60 (1), 1-20. doi: 10.1146/annurev-phyto-021621-115027
Templeton, J. M., Botella, J. R. and Blackall, P. J. (2022). Are point of management assays relevant for food safety in the poultry industries?. Microbiology Australia, 43 (2), 67-70. doi: 10.1071/MA22025
Yang, Fengbo, Miao, Yuchen, Liu, Yuyue, Botella, Jose R., Li, Weiqiang, Li, Kun and Song, Chun-Peng (2022). Function of protein kinases in leaf senescence of plants. Frontiers in Plant Science, 13 864215, 1-16. doi: 10.3389/fpls.2022.864215
Wei, Chunjie, Liu, Hao, Wang, Wenwen, Luo, Pengyu, Chen, Qiuling, Li, Rou, Wang, Chong, Botella, José Ramón and Zhang, Hui (2022). Expanding the editing window of cytidine base editors with the Rad51 DNA-binding domain in rice. Frontiers in Plant Science, 13 865848, 1-9. doi: 10.3389/fpls.2022.865848
Wang, Yijie and Botella, Jose Ramón (2022). Heterotrimeric G protein signaling in abiotic stress. Plants, 11 (7) 876, 876. doi: 10.3390/plants11070876
Li, Kun, Jia, Qianyun, Guo, Jinggong, Zhu, Zhijuan, Shao, Mengying, Wang, Jin, Li, Weiqiang, Dai, Jie, Guo, Minghui, Li, Rui, Song, Jieqiong, Yang, Fengbo, Du, Jingyi, Botella, Jose R., Song, Chun-Peng and Miao, Yuchen (2022). The high chlorophyll fluorescence 244 (HCF244) Is potentially involved in glutathione peroxidase 7-regulated high light stress in Arabidopsis thaliana. Environmental and Experimental Botany, 195 104767, 104767. doi: 10.1016/j.envexpbot.2021.104767
Chen, Qing‐Bin, Wang, Wen‐Jing, Zhang, Yue, Zhan, Qi‐Di, Liu, Kang, Botella, José Ramón, Bai, Ling and Song, Chun‐Peng (2022). Abscisic acid-induced cytoplasmic translocation of constitutive photomorphogenic 1 enhances reactive oxygen species accumulation through the HY5-ABI5 pathway to modulate seed germination. Plant, Cell and Environment, 45 (5), 1474-1489. doi: 10.1111/pce.14298
Gong, Zheng, Cheng, Ming and Botella, Jose R. (2021). Non-GM genome editing approaches in crops. Frontiers in Genome Editing, 3, 817279. doi: 10.3389/fgeed.2021.817279
Massel, Karen, Lam, Yasmine, Hintzsche, Jessica, Lester, Nicholas, Botella, Jose R. and Godwin, Ian D. (2021). Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals. Plant Cell Reports, 41 (2), 489-492. doi: 10.1007/s00299-021-02816-z
Maruta, Natsumi, Trusov, Yuri, Jones, Alan M. and Botella, Jose R. (2021). Heterotrimeric G proteins in plants: canonical and atypical Gα subunits. International Journal of Molecular Sciences, 22 (21) 11841, 11841. doi: 10.3390/ijms222111841
Ninh, Thi Thao, Gao, Wei, Trusov, Yuri, Zhao, Jing‐Ruo, Long, Lu, Song, Chun‐Peng and Botella, Jose Ramon (2021). Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses. Plant Direct, 5 (11) e359, 1-17. doi: 10.1002/pld3.359
Tao, Yongfu, Trusov, Yuri, Zhao, Xianrong, Wang, Xuemin, Cruickshank, Alan W., Hunt, Colleen, van Oosterom, Erik J., Hathorn, Adrian, Liu, Guoquan, Ian, Godwin D., Botella, Jose R., Mace, Emma S. and Jordan, David R. (2021). Manipulating assimilate availability provides insight into the genes controlling grain size in sorghum. The Plant Journal, 108 (1) tpj.15437, 1-13. doi: 10.1111/tpj.15437
Maruta, Natsumi, Trusov, Yuri, Urano, Daisuke, Chakravorty, David, Assmann, Sarah M., Jones, Alan M. and Botella, Jose R. (2021). GTP binding by Arabidopsis extra-large G protein 2 is not essential for its functions. Plant Physiology, 186 (2), 1-14. doi: 10.1093/plphys/kiab119
Zhan, Xiangqiang, Lu, Yuming, Zhu, Jian-Kang and Botella, Jose Ramon (2021). Genome editing for plant research and crop improvement. Journal of Integrative Plant Biology, 63 (1), 3-33. doi: 10.1111/jipb.13063
Mao, Yanfei, Wang, Mugui, Zhao, Yuhang, Huang, Boyu, Wu, Qingbing, Zheng, Qijie, Botella, Jose Ramon and Zhu, Jian-Kang (2021). Expanding the target range of base editing in plants without loss of efficiency by blocking RNA-silencing. Plant Biotechnology Journal, 19 (12), 2389-2391. doi: 10.1111/pbi.13712
Chen, Qingbin, Bai, Ling, Wang, Wenjing, Shi, Huazhong, Botella, José Ramón, Zhan, Qidi, Liu, Kang, Yang, Hong‐Quan and Song, Chun‐Peng (2020). COP1 promotes ABA‐induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases. New Phytologist, 229 (4) nph.17001, 2035-2049. doi: 10.1111/nph.17001
Mason, Michael G. and Botella, José R. (2020). Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks. Nature Protocols, 15 (11), 3663-3677. doi: 10.1038/s41596-020-0392-7
Zou, Yiping, Mason, Michael Glenn and Botella, Jose Ramon (2020). Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics. PLoS One, 15 (6) e0235216, e0235216. doi: 10.1371/journal.pone.0235216
Qin, Xiaohui, Duan, Zhikun, Zheng, Yuan, Liu, Wen-Cheng, Guo, Siyi, Botella, José Ramón and Song, Chun-Peng (2020). ABC1K10a, an atypical kinase, functions in plant salt stress tolerance. BMC Plant Biology, 20 (1) 270, 270. doi: 10.1186/s12870-020-02467-4
Gao, Wei, Xu, Fu‐Chun, Long, Lu, Li, Yang, Zhang, Jun‐Li, Chong, Leelyn, Botella, Jose Ramon and Song, Chun‐Peng (2020). The gland localized CGP1 controls gland pigmentation and gossypol accumulation in cotton. Plant Biotechnology Journal, 18 (7) pbi.13323, 1-12. doi: 10.1111/pbi.13323
Mason, M.G., Blackall, P.J., Botella, J.R. and Templeton, J.M. (2019). An easy‐to‐perform, culture‐free Campylobacter point‐of‐management assay for processing plant applications. Journal of Applied Microbiology, 128 (3) jam.14509, 620-629. doi: 10.1111/jam.14509
Maruta, Natsumi, Trusov, Yuri, Chakravorty, David, Urano, Daisuke, Assmann, Sarah M. and Botella, Jose R. (2019). Nucleotide exchange–dependent and nucleotide exchange–independent functions of plant heterotrimeric GTP-binding proteins. Science Signaling, 12 (606) eaav9526, eaav9526. doi: 10.1126/scisignal.aav9526
Mason, Michael Glenn and Botella, José Ramón (2019). A simple, robust and equipment-free DNA amplification readout in less than 30 seconds. RSC Advances, 9 (42), 24440-24450. doi: 10.1039/c9ra04725e
Hua, Kai, Zhang, Jinshan, Botella, Jose Ramon, Ma, Changle, Kong, Fanjiang, Liu, Baohui and Zhu, Jian-Kang (2019). Perspectives on the Application of Genome-Editing Technologies in Crop Breeding. Molecular Plant, 12 (8), 1047-1059. doi: 10.1016/j.molp.2019.06.009
Wang, Wenjing, Chen, Qingbin, Botella, Jose Ramon and Guo, Siyi (2019). Beyond light: insights into the role of constitutively photomorphogenic1 in plant hormonal signaling. Frontiers in Plant Science, 10 557, 557. doi: 10.3389/fpls.2019.00557
Mao, Yanfei, Botella, Jose Ramon, Liu, Yaoguang and Zhu, Jian-Kang (2019). Gene editing in plants: Progress and challenges. National Science Review, 6 (3), 421-437. doi: 10.1093/nsr/nwz005
Liu, Hao, Guo, Siyi, Lu, Minghua, Zhang, Yu, Li, Junhua, Wang, Wei, Wang, Pengtao, Zhang, Junli, Hu, Zhubing, Li, Liangliang, Si, Lingyu, Zhang, Jie, Qi, Qi, Jiang, Xiangning, Botella, José Ramón, Wang, Hua and Song, Chun-Peng (2019). Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment. Nature Communications, 10 (1) 1768, 1768. doi: 10.1038/s41467-019-09467-5
Botella, Jose R. (2019). Now for the hard ones: is there a limit on CRISPR genome editing in crops?. Journal of Experimental Botany, 70 (3), 734-737. doi: 10.1093/jxb/erz007
Feng, Zhengyan, Zhang, Zhengjing, Hua, Kai, Gao, Xifeng, Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2018). A highly efficient cell division-specific CRISPR/Cas9 system generates homozygous mutants for multiple genes in Arabidopsis. International Journal of Molecular Sciences, 19 (12) 3925, 3925. doi: 10.3390/ijms19123925
Long, Lu, Guo, Dan-Dan, Gao, Wei, Yang, Wen-Wen, Hou, Li-Pan, Ma, Xiao-Nan, Miao, Yu-Chen, Botella, Jose Ramon and Song, Chun-Peng (2018). Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression. Plant Methods, 14 (1) 85, 85. doi: 10.1186/s13007-018-0353-0
Mao, Yanfei, Yang, Xiaoxuan, Zhou, Yiting, Zhang, Zhengjing, Botella, Jose Ramon and Zhu, Jian-Kang (2018). Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 system. Genome Biology, 19 (1) 149, 149. doi: 10.1186/s13059-018-1529-7
Li, Haipeng, Li, Kun, Guo, Yutao, Guo, Jinggong, Miao, Kaiting, Botella, Jose R., Song, Chun-Peng and Miao, Yuchen (2018). A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum). Plant Methods, 14 (1) 50, 50. doi: 10.1186/s13007-018-0319-2
Guo, Jinggong, Li, Kun, Jin, Lifeng, Xu, Rui, Miao, Kaiting, Yang, Fengbo, Qi, Chaoya, Zhang, Lin, Botella, Jose R., Wang, Ran and Miao, Yuchen (2018). A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants. Plant Methods, 14 (1) 40, 40. doi: 10.1186/s13007-018-0305-8
Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2018). Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds (PLoS biology (2017) 15 11 (e2003916)). PLoS Biology, 16 (5). doi: 10.1371/journal.pbio.1002630
Zhang, Jinshan, Zhang, Hui, Botella, José Ramón and Zhu, Jian-Kang (2018). Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties. Journal of Integrative Plant Biology, 60 (5), 369-375. doi: 10.1111/jipb.12620
Gao, Wei, Xu, Fu-Chun, Guo, Dan-Dan, Zhao, Jing-Ruo, Liu, Ji, Guo, Ya-Wei, Singh, Prashant Kumar, Ma, Xiao-Nan, Long, Lu, Botella, Jose Ramon and Song, Chun-Peng (2018). Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress. BMC Plant Biology, 18 (1) 15, 1-15. doi: 10.1186/s12870-018-1230-8
Zhang, Hui, Zhang, Jinshan, Lang, Zhaobo, Botella, José Ramón and Zhu, Jian-Kang (2017). Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement. Critical Reviews in Plant Sciences, 36 (4), 291-309. doi: 10.1080/07352689.2017.1402989
Lau, Han Yih and Botella, Jose R. (2017). Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection. Frontiers in Plant Science, 8 2016, 2016. doi: 10.3389/fpls.2017.02016
Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2017). Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biology, 15 (11) e2003916, e2003916. doi: 10.1371/journal.pbio.2003916
Voss-Fels, Kai P., Robinson, Hannah, Mudge, Stephen R., Richard, Cecile, Newman, Saul, Wittkop, Benjamin, Stahl, Andreas, Friedt, Wolfgang, Frisch, Matthias, Gabur, Iulian, Miller-Cooper, Anika, Campbell, Bradley C., Kelly, Alison, Fox, Glen, Christopher, Jack, Christopher, Mandy, Chenu, Karine, Franckowiak, Jerome, Mace, Emma S., Borrell, Andrew K., Eagles, Howard, Jordan, David R., Botella, Jose R., Hammer, Graeme, Godwin, Ian D., Trevaskis, Ben, Snowdon, Rod J. and Hickey, Lee T. (2017). VERNALIZATION1 modulates root system architecture in wheat and barley. Molecular Plant, 11 (1), 226-229. doi: 10.1016/j.molp.2017.10.005
Tiwari, Ila Mukul, Jesuraj, Arun, Kamboj, Richa, Devanna, B. N., Botella, Jose R. and Sharma, T. R. (2017). Host Delivered RNAi, an efficient approach to increase rice resistance to sheath blight pathogen (Rhizoctonia solani). Scientific Reports, 7 (1) 7521, 7521. doi: 10.1038/s41598-017-07749-w
Gao, Wei, Long, Lu, Tian, Xinquan, Xu, Fuchun, Liu, Ji, Singh, Prashant K., Botella, Jose R. and Song, Chunpeng (2017). Genome editing in cotton with the CRISPR/Cas9 system. Frontiers in Plant Science, 8 1364, 1364. doi: 10.3389/fpls.2017.01364
Tao, Yongfu, Mace, Emma S., Tai, Shuaishuai, Cruickshank, Alan, Campbell, Bradley C., Zhao, Xianrong, Van Oosterom, Erik J., Godwin, Ian D., Botella, Jose R. and Jordan, David R. (2017). Whole-genome analysis of candidate genes associated with seed size and weight in Sorghum bicolor reveals signatures of artificial selection and insights into parallel domestication in cereal crops. Frontiers in Plant Science, 8 1237, 1237.1-1237.14. doi: 10.3389/fp/s.2017.01237
Zhang, Hui, Zhang, Jinshan, Yan, Jun, Gou, Feng, Mao, Yanfei, Tang, Guiliang, Botella, Jose Ramon and Zhu, Jian-Kang (2017). Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits. Proceedings of the National Academy of Sciences, 114 (20), 5277-5282. doi: 10.1073/pnas.1703752114
Richa, Kamboj, Tiwari, Ila M., Devanna, B. N., Botella, Jose R., Sharma, Vinay and Sharma, Tilak R. (2017). Novel chitinase gene LOC_Os11g47510 from indica rice tetep provides enhanced resistance against sheath blight pathogen Rhizoctonia solani in rice. Frontiers in Plant Science, 8 596, 596. doi: 10.3389/fpls.2017.00596
Wang, Mugui, Lu, Yuming, Botella, Jose Ramon, Mao, Yanfei, Hua, Kai and Zhu, Jian-kang (2017). Gene targeting by homology-directed repair in rice using a geminivirus-based CRISPR/Cas9 system. Molecular Plant, 10 (7), 1007-1010. doi: 10.1016/j.molp.2017.03.002
Lau, Han Yih, Wu, Haoqi, Wee, Eugene J. H., Trau, Matt, Wang, Yuling and Botella, Jose R. (2017). Specific and sensitive isothermal electrochemical biosensor for plant pathogen DNA detection with colloidal gold nanoparticles as probes. Scientific Reports, 7 (1) 38896, 38896. doi: 10.1038/srep38896
Massel, Karen, Campbell, Bradley C., Mace, Emma S., Tai, Shuaishuai, Tao, Yongfu, Worland, Belinda G., Jordan, David R., Botella, Jose R. and Godwin, Ian D. (2016). Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor. Frontiers in Plant Science, 7 (OCTOBER2016) 1544, 1544. doi: 10.3389/fpls.2016.01544
Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2016). Heritability of targeted gene modifications induced by plant-optimized CRISPR systems. Cellular and Molecular Life Sciences, 74 (6), 1-19. doi: 10.1007/s00018-016-2380-1
Urano, Daisuke, Maruta, Natsumi, Trusov, Yuri, Stoian, Richard, Wu, Qingyu, Liang, Ying, Jaiswal, Dinesh Kumar, Thung, Leena, Jackson, David, Botella, José Ramón and Jones, Alan M. (2016). Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom. Science Signaling, 9 (446) ra93, ra93-ra93. doi: 10.1126/scisignal.aaf9558
Lau, Han Yih, Wang, Yuling, Wee, Eugene J. H., Botella, Jose R. and Trau, Matt (2016). Field demonstration of a multiplexed point-of-care diagnostic platform for plant pathogens. Analytical Chemistry, 88 (16), 8074-8081. doi: 10.1021/acs.analchem.6b01551
Brenya, Eric, Trusov, Yuri, Dietzgen, Ralf Georg and Botella, Jose Ramon (2016). Heterotrimeric G-proteins facilitate resistance to plant pathogenic viruses in Arabidopsis thaliana (L.) Heynh. Plant Signaling and Behavior, 11 (8) e1212798, e1212798. doi: 10.1080/15592324.2016.1212798
Trusov, Yuri and Botella, Jose R. (2016). Plant G-proteins come of age: breaking the bond with animal models. Frontiers in Chemistry, 4 (24) 24, 1-9. doi: 10.3389/fchem.2016.00024
Richa, Kamboj, Tiwari, Ila M., Kumari, Mandeep, Devanna, B. N., Sonah, Humira, Kumari, Archana, Nagar, Ramawatar, Sharma, Vinay, Botella, Jose R. and Sharma, Tilak R. (2016). Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep. Frontiers in Plant Science, 7 (244) 244, 1-10. doi: 10.3389/fpls.2016.00244
Subramaniam, Gayathery, Trusov, Yuri, Lopez-Encina, Carlos, Hayashi, Satomi, Batley, Jacqueline and Botella, Jose Ramon (2016). Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato. Plant Physiology, 170 (2), 1117-1134. doi: 10.1104/pp.15.01675
Botella, Jose R. and Botella, Miguel A. (2016). Preface. Methods in Molecular Biology, 1363, vii-x.
Zhang, Zhengjing, Mao, Yanfei, Ha, Si, Liu, Wenshan, Botella, Jose Ramon and Zhu, Jian-Kang (2015). A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis. Plant Cell Reports, 35 (7), 1-15. doi: 10.1007/s00299-015-1900-z
Aranda-Sicilia, Maria Nieves, Trusov, Yuri, Maruta, Natsumi, Chakravorty, David, Zhang, Yuelin and Botella, Jose Ramon (2015). Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis. Journal of Plant Physiology, 188, 44-48. doi: 10.1016/j.jplph.2015.09.005
Liu, Wenshan, Zhu, Xiaohong, Lei, Mingguang, Xia, Qingyou, Botella, Jose Ramon, Zhu, Jian-Kang and Mao, Yanfei (2015). A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana. Science Bulletin, 60 (15), 1332-1347. doi: 10.1007/s11434-015-0848-2
Zhang, Hui, Gou, Feng, Zhang, Jinshan, Liu, Wenshan, Li, Qianqian, Mao, Yanfei, Botella, Jose Ramon and Zhu, Jian-Kang (2015). TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice. Plant Biotechnology Journal, 14 (1), 186-194. doi: 10.1111/pbi.12372
Maruta, Natsumi, Trusov, Yuri, Brenya, Eric, Parekh, Urvi and Botella, Jose Ramon (2015). Membrane-localized extra-large G proteins and Gβγ of the heterotrimeric G proteins form functional complexes engaged in plant immunity in arabidopsis. Plant Physiology, 167 (3), 1004-1016. doi: 10.1104/pp.114.255703
Hu, Zongli, Parekh, Urvi, Maruta, Natsumi, Trusov, Yuri and Botella, Joser R. (2015). Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance. Frontiers in Chemistry, 3 (JAN) 1, 1. doi: 10.3389/fchem.2015.00001
Wee, E. J. H., Lau, H. Y., Botella, J. and Trau, M. (2015). Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings. Chemical Communications, 51 (27), 5828-5831. doi: 10.1039/C4CC10068A
Mao, Yanfei, Zhang, Zhengjing, Feng, Zhengyan, Wei, Pengliang, Zhang, Hui, Botella, Jose Ramon and Zhu, Jian-Kang (2015). Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis. Plant Biotechnology Journal, 14 (2), 519-532. doi: 10.1111/pbi.12468
Wolfenstetter, Susanne, Chakravorty, David, Kula, Ryan, Urano, Daisuke, Trusov, Yuri, Sheahan, Michael B., McCurdy, David W., Assmann, Sarah M., Jones, Alan M. and Botella, Jose R. (2014). Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis. Plant Journal, 81 (3), 388-398. doi: 10.1111/tpj.12732
Moyle, Richard L., Koia, Jonni H., Vrebalov, Julia, Giovannoni, James and Botella, Jose R. (2014). The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant. Plant Molecular Biology, 86 (4-5), 395-407. doi: 10.1007/s11103-014-0236-3
Lau, Han Yih, Palanisamy, Ramkumar, Trau, Matt and Botella, Jose R. (2014). Molecular inversion probe: a new tool for highly specific detection of plant pathogens. PLoS One, 9 (10) e111182, e111182.1-e111182.9. doi: 10.1371/journal.pone.0111182
Moyle, Richard L. and Botella, Jose R. (2014). EST sequencing of Meloidogyne javanica infected pineapple root tissues reveals changes in gene expression during root-knot nematode induced gall formation. Tropical Plant Biology, 7 (2), 43-52. doi: 10.1007/s12042-014-9136-6
Liu, Jinman, Ding, Pingtao, Sun, Tongjun, Nitta, Yukino, Dong, Oliver, Huang, Xingchuan, Yang, Wei, Li, Xin, Botella, Jose Ramon and Zhang, Yuelin (2013). Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases. Plant Physiology, 161 (4), 2146-2158. doi: 10.1104/pp.112.212431
Urano, Daisuke, Chen, Jin-Gui, Botella, Jose Ramon and Jones, Alan M. (2013). Heterotrimeric G protein signalling in the plant kingdom. Open Biology, 3 (MAR) 120186, 1-22. doi: 10.1098/rsob.120186
Koia, Jonni, Moyle, Richard, Hendry, Caroline, Lim, Lionel and Botella, Jose Ramon (2013). Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana. Plant Molecular Biology, 81 (4-5), 327-336. doi: 10.1007/s11103-012-0002-3
Thung, Leena, Chakravorty, David, Trusov, Yuri, Jones, Alan M. and Botella, Jose Ramon (2013). Signaling specificity provided by the Arabidopsis thaliana Heterotrimeric G-Protein gamma subunits AGG1 and AGG2 is partially but not exclusively provided through transcriptional regulation. PLoS One, 8 (3) e58503, e58503.1-e58503.11. doi: 10.1371/journal.pone.0058503
Koia, Jonni H., Moyle, Richard L. and Botella, Jose R. (2012). Microarray analysis of gene expression profiles in ripening pineapple fruits. BMC Plant Biology, 12 (1) 240, 240.1-240.13. doi: 10.1186/1471-2229-12-240
Trusov, Yuri and Botella, Jose Ramon (2012). New faces in plant innate immunity: heterotrimeric G proteins. Journal of Plant Biochemistry and Biotechnology, 21 (1), S40-S47. doi: 10.1007/s13562-012-0140-3
Botella, Jose Ramon (2012). Can heterotrimeric G proteins help to feed the world?. Trends in Plant Science, 17 (10), 563-568. doi: 10.1016/j.tplants.2012.06.002
Jiang, Kun, Frick-Cheng, Arwen, Trusov, Yuri, Delgado-Cerezo, Magdalena, Rosenthal, David M., Lorek, Justine, Panstruga, Ralph, Booker, Fitzgerald L., Botella, Jose Ramon, Molina, Antonio, Ort, Donald R. and Jones, Alan M. (2012). Dissecting Arabidopsis Gβ signal transduction on the protein surface1[W][OA]. Plant Physiology, 159 (3), 975-983. doi: 10.1104/pp.112.196337
Thung, Leena, Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012). G gamma 1+G gamma 2+G gamma 3=G beta: The search for heterotrimeric G-protein gamma subunits in Arabidopsis is over. Journal of Plant Physiology, 169 (5), 542-545. doi: 10.1016/j.jplph.2011.11.010
Chakravorty, David, Trusov, Yuri and Botella, Jose Ramon (2012). Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein beta subunit suggests divergent mechanisms of effector activation between plant and animal G proteins. Planta, 235 (3), 615-627. doi: 10.1007/s00425-011-1526-5
Trusov, Yuri, Chakravorty, David and Botella, Jose Ramon (2012). Diversity of heterotrimeric G-protein subunits in plants. BMC Research Notes, 5 (1) 608, 608.1-608.9. doi: 10.1186/1756-0500-5-608
McCallum, Emily J., Cunningham, John Paul, Lücker, Joost, Zalucki, Myron P., De Voss, James J . and Botella, José R. (2011). Increased plant volatile production affects oviposition, but not larval development, in the moth Helicoverpa armigera. Journal of Experimental Biology, 214 (21), 3672-3677. doi: 10.1242/jeb.059923
Chakravorty, David, Trusov, Yuri, Zhang, Wei, Acharya, Biswa R., Sheahan, Michael B., McCurdy, David W., Assmann, Sarah M. and Botella, José Ramón (2011). An atypical heterotrimeric G-protein gamma-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana. Plant Journal, 67 (5), 840-851. doi: 10.1111/j.1365-313X.2011.04638.x
Klopffleisch, Karsten, Phan, Nguyen, Augustin, Kelsey, Bayne, Robert S., Booker, Katherine S., Botella, Jose R., Carpita, Nicholas C., Carr, Tyrell, Chen, Jin-Gui, Cooke, Thomas Ryan, Frick-Cheng, Arwen, Friedman, Erin J., Fulk, Brandon, Hahn, Michael G., Jiang, Kun, Jorda, Lucia, Kruppe, Lydia, Liu, Chenggang, Lorek, Justine, McCann, Maureen C., Molina, Antonio, Moriyama, Etsuko N., Mukhtar, M. Shahid, Mudgil, Yashwanti, Pattathil, Sivakumar, Schwarz, John, Seta, Steven, Tan, Matthew, Temp, Ulrike ... Jones, Alan M. (2011). Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. Molecular Systems Biology, 7 (1) 532, 532.1-532.7. doi: 10.1038/msb.2011.66
Wever, Willem, McCallum, Emily J., Chakravorty, David, Cazzonelli, Christopher I. and Botella, José R. (2010). The 5' untranslated region of the VR-ACS1 mRNA acts as a strong translational enhancer in plants. Transgenic Research, 19 (4), 667-674. doi: 10.1007/s11248-009-9332-6
O'Connell, A, Robin, G, Kobe, B and Botella, JR (2009). Biochemical characterization of arabidopsis developmentally regulated G-proteins (DRGs). PROTEIN EXPRESSION AND PURIFICATION, 67 (2), 88-95. doi: 10.1016/j.pep.2009.05.009
Trusov, Yuri, Sewelam, Nasser, Rookes, James Edward, Kunkel, Matt, Nowak, Ekaterina, Schenk, Peer Martin and Botella, Jose R (2009). Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling. The Plant Journal, 58 (1), 69-81. doi: 10.1111/j.1365-313X.2008.03755.x
Mendoza, E.M.T., Laurena, A.C. and Botella, J.R. (2008). Recent advances in the development of transgenic papaya technology. Biotechnology Annual Review, 14, 423-461. doi: 10.1016/S1387-2656(08)00019-7
Trusov, Y., Zhang, W., Assmann, S. M. and Botella, J. R. (2008). G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants. Plant Physiology, 147 (2), 636-649. doi: 10.1104/pp.108.117655
Naponelli, V., Noiriel, A., Ziemak, M.J., Beverley, S.M., Lye, L-F., Plume, A.M., Botella, J.R., Loizeau, K., Ravanel, S., Rébeillé, F., de Crécy-Lagard, V. and Hanson, A.D. (2008). Phylogenomic and Functional Analysis of Pterin-4a-Carbinolamine Dehydratase Family (COG2154) Proteins in Plants and Microorganisms. Plant Physiology, 146 (4), 1515-1527. doi: 10.1104/pp.107.114090
Purnell, Matthew P., Skopelitis, Damianos S., Roubelakis-Angelakis, Kalliopi A. and Botella, José R. (2007). Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels. Planta, 225 (5), 1325-1325. doi: 10.1007/s00425-007-0481-7
Fairbairn, David J., Cavallaro, Antonino S., Bernard, Margaret, Mahalinga-Iyer, Janani, Graham, Michael W. and Botella, José R. (2007). Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes. Planta, 226 (6), 1525-1533. doi: 10.1007/s00425-007-0588-x
Anderson, D. J. and Botella, J. R. (2007). Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1. Plant Cell Reports, 26 (9), 1469-1480. doi: 10.1007/s00299-007-0356-1
Trusov, Yuri, Rookes, James Edward, Tilbrook, Kimberley, Chakravorty, David, Mason, Michael Glenn, Anderson, David, Chen, Jin-Gui, Jones, Alan M. and Botella, Jose Ramon (2007). Heterotrimeric G Protein γ Subunits Provide Functional Selectivity in Gßγ Dimer Signaling in Arabidopsis. Plant Cell, 19 (4), 1235-1250. doi: 10.1105/tpc.107.050096
Chakravorty, David and Botella, José Ramón (2007). Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and alpha subunit knockouts. Gene, 393 (1-2), 163-170. doi: 10.1016/j.gene.2007.02.008
Purnell, M. P. and Botella, J. R. (2007). Tobacco isoenzyme 1 of NAD(H)-dependent glutamate dehydrogenase catabolizes glutamate in vivo. Plant Physiology, 143 (1), 530-539. doi: 10.1104/pp.106.091330
Trusov, Y. and Botella, J. R. (2006). Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]. Journal of Experimental Botany, 57 (14), 3953-3960. doi: 10.1093/jxb/erl167
Trusov, Y., Rookes, J. E., Chakravorty, D., Armour, D., Schenk, P. M. and Botella, J. R. (2006). Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiology, 140 (1), 210-220. doi: 10.1104/pp.105.069625
Cazzonelli, Christopher I., McCallum, Emily J., Lee, Rebecca and Botella, Jose Ramon (2005). Characterization of a strong, constitutive mung bean (Vigna radiata L.) promoter with a complex mode of regulation in planta. Transgenic Research, 14 (6), 941-967. doi: 10.1007/s11248-005-2539-2
Purnell, M. P., Skopelitis, D. S., Roubelakis-Angelakis, K. A. and Botella, Jose R. (2005). Modulation of higher-plant NAD(H)-dependent glutamate dehydrogenase activity in transgenic tobacco via alteration of beta subunit levels. Planta, 222 (1), 167-180. doi: 10.1007/s00425-005-1510-z
Botella, J. R. (2005). Balancing teaching and research in higher education. Studies in Learning Evaluation Innovation and Development, 2 (2), 3-4.
Moyle, R. L., Ripi-Koia, J., Crowe, M. L., Fairbairn, D. J. and Botella, J. R. (2005). PineappleDB: An online pineapple bioinformatics resource. BMC Plant Biology, 5 (21) 21, 1-5. doi: 10.1186/1471-2229-5-21
Hidalgo, M. S. P., Tecson-Mendoza, E. M., Laurena, A. C. and Botella, J. R. (2005). Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms. Journal of Biochemistry And Molecular Biology, 38 (3), 320-327. doi: 10.5483/bmbrep.2005.38.3.320
Moyle, Richard, Fairbairn, David J., Ripi, Jonni, Crowe, Mark and Botella, Jose R. (2005). Developing pineapple fruit has a small transcriptome dominated by metallothionein. Journal of Experimental Botany, 56 (409), 101-112. doi: 10.1093/jxb/eri015
Petsch, K. A., Botella, J. R. and Mylne, J. (2005). Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division. Plant Physiology, 139 (1), 115-126. doi: 10.1104/pp.105.062695
Kim, J. H. and Botella, J. R. (2004). Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation. Plant Cell Tissue And Organ Culture, 78 (1), 69-73. doi: 10.1023/B:TICU.0000020396.64257.c1
Gonzalez, N. and Botella, J. R. (2003). Characterisation of three ACC synthase gene family members during post-harvest-induced senescence in broccoli (Brassica oleracea L. var. italica). Journal of Plant Biology, 46 (4), 223-230. doi: 10.1007/BF03030368
Kim, J. H and Botella, J. R. (2002). Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation. Journal of Plant Biology, 45 (3), 177-181. doi: 10.1007/BF03030311
Mason, Michael G. and Botella, José R. (2001). Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with G beta. Biochimica et biophysica acta (BBA). Gene structure and expression, 1520 (2), 147-153. doi: 10.1016/S0167-4781(01)00262-7
Humphrey, Tania Vivienne and Botella, José Ramón (2001). Re-evaluation of the cytokinin receptor role of the Arabidopsis gene GCR1. Journal of Plant Physiology, 158 (5), 645-653. doi: 10.1078/0176-1617-00316
Encina, CL, Constantin, M and Botella, J (2001). An easy and reliable method for establishment and maintenance of leaf and root cell cultures of Arabidopsis thaliana. Plant Molecular Biology Reporter, 19 (3), 245-248. doi: 10.1007/BF02772896
Botella, J. R. and Mason, M. (2000). Completing the heterotrimer: Isolation and characterization of an Arabidopsis thaliana G Protein -subunit cDNA. Proceedings of the National Academy of Sciences of the United States of America, 97 (26), 14784-14788. doi: 10.1073/pnas.97.26.14784
Etheridge, N, Trusov, Y, Verbelen, JP and Botella, JR (1999). Characterization of ATDRG1, a member of a new class of GTP-binding proteins in plants. Plant Molecular Biology, 39 (6), 1113-1126. doi: 10.1023/A:1006137221259
Mylne, J and Botella, JR (1998). Binary vectors for sense and antisense expression of arabidopsis ESTs. Plant Molecular Biology Reporter, 16 (3), 257-262. doi: 10.1023/A:1007520410190
Cazzonelli, CI, Cavallaro, AS and Botella, JR (1998). Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits. Australian Journal of Plant Physiology, 25 (5), 513-518. doi: 10.1071/PP98013
Mylne, Joshua S. , Mason, Michael G. and Botella, José R. (1998). Rapid isolation of high-quality RNA from symbiotic dinoflagellates. Phycologia, 37 (4), 307-309. doi: 10.2216/i0031-8884-37-4-307.1
Purnell, MP, Stewart, GR and Botella, JR (1997). Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.). Gene, 186 (2), 249-254. doi: 10.1016/S0378-1119(96)00716-0
Mason, Michael Glenn and Botella, José Ramón (1997). Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening. Australian Journal of Plant Physiology, 24 (2), 239-244. doi: 10.1071/PP96111
Botella, JR, Arteca, JM, Somodevilla, M and Arteca, RN (1996). Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata). Plant Molecular Biology, 30 (6), 1129-1137. doi: 10.1007/BF00019547
Botella, JR, Arteca, RN and Frangos, JA (1995). A mechanical strain-induced 1-aminocyclopropane-1-carboxylic acid synthase gene. Proceedings of the National Academy of Sciences of the United States of America, 92 (5), 1595-1598. doi: 10.1073/pnas.92.5.1595
Thoma, S, Hecht, U, Kippers, A, Botella, J, Devries, S and Somerville, C (1994). Tissue-Specific Expression of a Gene Encoding a Cell Wall-Localized Lipid Transfer Protein From Arabidopsis. Plant Physiology, 105 (1), 35-45. doi: 10.1104/pp.105.1.35
Botella, JR and Arteca, RN (1994). Differential expression of two calmodulin genes in response to physical and chemical stimuli. Plant Molecular Biology, 24 (5), 757-766. doi: 10.1007/BF00029857
Botella, JR, Schlagnhaufer, CD, Arteca, JM, Arteca, RN and Phillips, AT (1993). Identification of two new members of the l-aminocyclopropane-l-carboxylate synthase-encoding multigene family in mung bean. Gene, 123 (2), 249-253. doi: 10.1016/0378-1119(93)90132-M
Botella, JR, Arteca, JM, Schlagnhaufer, CD, Arteca, RN and Phillips, AT (1992). Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid. Plant Molecular Biology, 20 (3), 425-436. doi: 10.1007/BF00040602
Botella, JR, Schlagnhaufer, CD, Arteca, RN and Phillips, AT (1992). Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments. Plant Molecular Biology, 18 (4), 793-797. doi: 10.1007/BF00020022
Bernhard, WR, Thoma, S, Botella, J and Somerville, CR (1991). Isolation of a Cdna Clone for Spinach Lipid Transfer Protein and Evidence That the Protein Is Synthesized by the Secretory Pathway. Plant Physiology, 95 (1), 164-170. doi: 10.1104/pp.95.1.164
Valpuesta, V, Perezrodriguez, MJ, Quesada, MA and Botella, JR (1989). 2 Polypeptides From Green Tomato Leaves Recognized by Antibodies Against Chloroplastic Glutamine-Synthetase. Plant Physiology and Biochemistry, 27 (6), 963-966.
BOTELLA, , VERBELEN, JP and VALPUESTA, (1988). IMMUNOCYTOLOCALIZATION OF GLUTAMINE-SYNTHETASE IN GREEN LEAVES AND COTYLEDONS OF LYCOPERSICON-ESCULENTUM. Plant Physiology, 88 (3), 943-946. doi: 10.1104/pp.88.3.943
Botella, JR, Verbelen, JP and Valpuesta, V (1988). Immunocytolocalization of ferredoxin-GOGAT in the cells of green leaves and cotyledons of Lycopersicon esculentum. Plant Physiology, 87 (1), 255-257. doi: 10.1104/pp.87.1.255
Avila, C, Botella, JR, Canovas, FM, Decastro, IN and Valpuesta, V (1987). Different Characteristics of the 2 Glutamate Synthases in the Green Leaves of Lycopersicon-Esculentum. Plant Physiology, 85 (4), 1036-1039. doi: 10.1104/pp.85.4.1036
Botella Mesa, J. R. and Verbelen, J. P. (1987). Immunocytochemical localization of glutamine synthetase in leaves of tomato seedlings. Micron And Microscopica Acta, 18 (3), 219-220. doi: 10.1016/0739-6260(87)90068-2
Botella, JR, Canovas, FM, Avila, C, Decastro, IN and Valpuesta, V (1986). Immunochemical Comparison of Glutamine Synthetases From Some Solanaceae Plants. Plant Physiology, 82 (2), 585-587. doi: 10.1104/pp.82.2.585
Canovas, FM, Avila, C, Botella, JR, Valpuesta, V and Decastro, IN (1986). Effect of Light-Dark Transition On Glutamine-Synthetase Activity in Tomato Leaves. Physiologia Plantarum, 66 (4), 648-652. doi: 10.1111/j.1399-3054.1986.tb05593.x
Conference Papers
Naseem, M.N., Omaleki, L., Templeton, J.M., Muralidhar, M., Botella, J.R., Blackall, P.J. and Turni, C. (2023). Use of piggery effluent for point-of-management diagnostics: A proof of concept. Manipulating Pig Production XIX, Brisbane, QLD Australia, 13-16 November 2023. Amsterdam, Netherlands: Elsevier. doi: 10.1016/j.anscip.2023.09.008
Moyle, R. L., Koia, J., Fairbairn, D. J. and Botella, J. R. (2006). The Pineapple EST sequencing and microarray project. The 5th International Pineapple Symposium, Port Alfred, South Africa, 11-16 April 2005. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2006.702.5
Trusov, Y. and Botella, J. R. (2006). Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene. The 5th International Pineapple Symposium, Port Alfred, South Africa, 11-16 April 2005. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2006.702.2
Botella, J. R. and Fairbairn, D. J. (2005). Present and future potential of pineapple biotechnology. Fourth International Pineapple Symposium, Veracruz, Mexico, 16-19 April 2002. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2005.666.1
Magdalita, P. M, Laurena, A. C., Yabut-Perez, B. M., Zaporteza, M. M., Tecson-Mendoza, E. M., Villegas, V. N. and Botella, J. R. (2003). Towards transformation, regeneration and screening of papaya containing antisense ACC synthase gene. 10th IAPTC&B Congress, Orlando, Florida, USA, 23-28 June, 2002. DORDRECHT: Springer.
Botella, J. R. (2003). What can molecular biology do for postharvest technology?. APEC Symposium on Postharvest Handling Systems, Radison Hotel, Bangkok, Thailand, 1-3 September, 2003.
Magdalita, P. M., Laurena, A. C., Yabut-Perez, B.M., Tecson-Mendoza, E. M., Villegas, V. N. and Botella, J. R. (2002). Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.17
Laurena, A. C., Magdalita, P. M., Hidalgo, M. S. P., Villegas, V. N., Mendoza, E. M. T. and Botella, J. R. (2002). Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.). The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.16
Botella, J. R. (2002). Bigger, firmer, riper. Biotechnological control of ripening in tropical fruits. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, 26 Nov - 1 Dec 2000. Leuven, Belgium: ISHS. doi: 10.17660/ActaHortic.2002.575.69
Cruz-Hernandez, A., Town, L. J., Cavallaro, A. S. and Botella, J. R. (2000). Transient and stable transformation in mango by particle bombardment. Sixth International Symposium on Mango, Pattaya City, Thailand, 6-9 April 1999. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2000.509.24
Humphrey, T. and Botella, J. R. (2000). Re-evaluating the receptor of the Arabidopsis gene GCR1. Com Bio 2000, Wellington Festival and Convention Center, NZ, 11-14 Dec, 2000. Victoria, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Ferris, R., Mylne, J. S. and Botella, J. R. (2000). Plentiful's Tale. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Purnell, M. P. and Botella, J. R. (2000). Is the hunt over? A role for the elusive enzyme GDH. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Victoria, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Botella, J. R., Leeton, P. R. J. and Cavallaro, A. S. (2000). Differential expression of the ACC Synthase and ACC Oxidase enzymes involved in fruit ripening in papaya. Combio 2000, Wellington Festival and Convention Center, NZ, 11-14 December 2000. Kent Town, SA: Australian Society for Biochemistry and Molecular Biology.
Petsch, K. A., Mylne, J. S. and Botella, J. R. (2000). Freaky Floral Bouquets! Over-expression of the 'Broomhead' gene in Arabidopsis. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Kent Town, S.A., Australia: Australian Society for Biochemistry and Molecular Biology.
Cavallaro, A. S., Leeton, P. R. J. and Botella, J. R. (2000). Biolistic Transformation of papaya cv sunrise solo. Combio 2000, Wellington Festival and Convention Center, NZ, 11-14 December 2000. Kent Town, SA: Australian Society for Biochemistry and Molecular Biology.
Mason, M. and Botella, J. R. (2000). Plant heterotrimeric G-Protein pathways exposed. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Etheridge, N. and Botella, J. R. (2000). The Arabidopsis member of the developmentally regulated GTP-binding proteins:AtDRG1. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Plume, A. M. and Botella, J. R. (2000). Characterisation of a novel G protein interactor in Arabidopsis. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Botella, J. R. (2000). Oh My God, we are eating genes! the myth and reality of GM Foods. ABA 2000 Biotechnology: Our Passport to the Next Millenium, Brisbane, 2-6 July, 2000. Brisbane Australia: Australian Biotechnology Association.
Anderson, D. and Botella, J. R. (2000). On the trail of G Proteins in plants. Com Bio 2000, Wellington, NZ, 11-14 Dec, 2000. Vic, Australia: Australian Society for Biochemistry and Molecular Biology, Inc.
Cazzonelli, C., Cavallaro, A. S. and Botella, J. R. (2000). Towards the production of transgenic pineapple to control flowering and ripening. 3rd International Pineapple Symposium, Pattaya, Thailand, 17-20 November 1998. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2000.529.13
Cazzonelli, C., Cavallaro, A. S. and Botella, J. R. (1999). Searching for the role of ethlene in non-climacteric fruits. Proceedings of the EU-TMR-Euro Conference Symp on Biol, Thira (Santorini), Greece, 5-8 Sept, 99. Dordrecht, The Netherlands: Kluwer Academic Publishers.
Botella, J. R., Cruz-Hernandez, A., Mason, M., Leeton, P. R. J., Cavallaro, A. S. and Labrie, P. (1999). Control of ripening in papaya and mango by genetic engineering. Int'l Symp on Plant Genetic Eng: Towards the Third Millen, Cuba, 6-10 Dec, 99. La Habana, Cuba: Elfos Scientiae.
Peters, KF, Grof, CPL, Botella, J and Albert, H (1996). Isolation and genetic manipulation of invertase genes in sugarcane. International Symposium on Sugar 2000, Brisbane Australia, Aug 19-23, 1996. EAST MELBOURNE: C S I R O.
Botella, JR, Arteca, JM, Somodevilla, M and Arteca, RN (1995). Mungbean Calcium-Dependent Protein-Kinase Cdna and its Expression in Response to Physical and Chemical Stimuli. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.
Botella, JR and Arteca, RN (1993). Differential Expression of 2 Calmodulin Genes in Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.
Arteca, JM, Botella, JR and Arteca, RN (1993). Effects of Plant Hormones On Acc Synthase Gene-Expression in Etiolated Mung Beans. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.
Botella, JR and Arteca, RN (1993). A Multigene Family for Protein-Kinases in Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.
Arteca, JM, Arteca, RN and Botella, JR (1993). Relationship Between Calcium and Ethylene Biosynthesis in Etiolated Mung Bean. ROCKVILLE: AMER SOC PLANT PHYSIOLOGISTS.
Patent
Botella, J. R., Graham, M. and Fairbairn, D. J. (2002). A method for facilitating pathogen resistance. 2002301927.